Isotype Control Flow Cytometry Gating

An ideal isotype control should.

Isotype control flow cytometry gating.

The section of the above paper focusing on isotype controls summarizes the problems with their use very clearly. Control sample type primary ab secondary reason cells only use treated and untreated cells. Naseem leeds institute of cardiovascular and metabolic medicine university of leeds leeds uk platelet flow cytometry is widely used in cardiovascular medicine as the platelet surface is rich in clinical bio markers. Considerations for the control of background fluorescence in clinical flow cytometry.

Considerations for the control of background fluorescence in clinical flow cytometry. Gating out dead cells using live dead marker isoclonic controls. Instrument setup controls and panel performance benjamin e j. Clinical cytometry 76 6 355 364.

This is where cells are stained in the presence of an excess of identical unlabeled antibody. As maecker and trotter state it is thus a hit or miss prospect to find an isotype control that truly matches the background staining of a particular test antibody. The article also illustrates difference in undesirable binding at different levels using the same clone from different manufacturers. Recommended controls for flow cytometry facs along with the samples to be labeled the following controls should be used whenever possible.

They should not be used to distinguish positive from negative cells or set positive gating regions. Alternatively you can use our handy search table located at the bottom of the page to find the right isotype control for your experiment. The various positive controls are used for compensating and gating when setting up the flow cytometer. Spurgeon and khalid m.

Gating out dead cells using live dead marker to see our range of flow cytometry isotype controls with information on how and when to use them download our isotype controls brochure.